Cagrilintide impurity profile
Every named species the index has reported alongside Cagrilintide, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
1.42%
n=32
Median largest single
0.74%
n=32
Worst single observed
1.83%
n=32
Named species observed
5 / 5
n=5
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| cagrilintide dimer (intermolecular disulfide) | +3.44 | +3959.306 | 100% | 0.11 | 1.08 |
| des-C16 diacid (−254 Da) | -3.62 | -254.225 | 97% | 0.29 | 1.07 |
| open-chain (reduced disulfide, +2 Da) | -1.21 | +2.016 | 94% | 0.14 | 1.83 |
| disulfide-scrambled isomer | +0.48 | mass-identical | 94% | 0.15 | 1.30 |
| deamidated (+1 Da) | -0.44 | +0.984 | 91% | 0.11 | 0.82 |
cagrilintide dimer (intermolecular disulfide)
- Retention offset from the main peak
- +3.44 min on RG-2
- Mass difference
- +3959.306 Da
- Reported in
- 32 of 32 records (100 %)
- Level when reported
- median 0.11 %, max 1.08 %
- Resolution requirement
- Baseline-resolved on the reference method.
des-C16 diacid (−254 Da)
- Retention offset from the main peak
- -3.62 min on RG-2
- Mass difference
- -254.225 Da
- Reported in
- 31 of 32 records (97 %)
- Level when reported
- median 0.29 %, max 1.07 %
- Resolution requirement
- Baseline-resolved on the reference method.
open-chain (reduced disulfide, +2 Da)
- Retention offset from the main peak
- -1.21 min on RG-2
- Mass difference
- +2.016 Da
- Reported in
- 30 of 32 records (94 %)
- Level when reported
- median 0.14 %, max 1.83 %
- Resolution requirement
- Baseline-resolved on the reference method.
disulfide-scrambled isomer
- Retention offset from the main peak
- +0.48 min on RG-2
- Mass difference
- mass-identical — chromatography only
- Reported in
- 30 of 32 records (94 %)
- Level when reported
- median 0.15 %, max 1.30 %
- Resolution requirement
- A gradient long enough to separate it. No mass spectrometer will.
deamidated (+1 Da)
- Retention offset from the main peak
- -0.44 min on RG-2
- Mass difference
- +0.984 Da
- Reported in
- 29 of 32 records (91 %)
- Level when reported
- median 0.11 %, max 0.82 %
- Resolution requirement
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- disulfide scrambling above pH 7.5 — the main stability liability
- aggregation of the open-chain form, which nucleates fibrils rapidly