L-Glutathione (reduced) impurity profile
Every named species the index has reported alongside L-Glutathione (reduced), how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
1.00%
n=122
Median largest single
0.50%
n=122
Worst single observed
2.05%
n=122
Named species observed
4 / 4
n=4
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| glutathione disulfide (GSSG, +305 Da) | +0.52 | +304.984 | 98% | 0.21 | 1.68 |
| S-sulfonate adduct (+80 Da) | -0.14 | +79.957 | 96% | 0.13 | 1.02 |
| γ-Glu-Cys (−57 Da) | -0.18 | -57.021 | 92% | 0.12 | 2.05 |
| cysteinyl-glycine (−129 Da) | -0.24 | -129.043 | 87% | 0.18 | 1.52 |
glutathione disulfide (GSSG, +305 Da)
- Retention offset from the main peak
- +0.52 min on RG-2
- Mass difference
- +304.984 Da
- Reported in
- 120 of 122 records (98 %)
- Level when reported
- median 0.21 %, max 1.68 %
- Resolution requirement
- Baseline-resolved on the reference method.
S-sulfonate adduct (+80 Da)
- Retention offset from the main peak
- -0.14 min on RG-2
- Mass difference
- +79.957 Da
- Reported in
- 117 of 122 records (96 %)
- Level when reported
- median 0.13 %, max 1.02 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
γ-Glu-Cys (−57 Da)
- Retention offset from the main peak
- -0.18 min on RG-2
- Mass difference
- -57.021 Da
- Reported in
- 112 of 122 records (92 %)
- Level when reported
- median 0.12 %, max 2.05 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
cysteinyl-glycine (−129 Da)
- Retention offset from the main peak
- -0.24 min on RG-2
- Mass difference
- -129.043 Da
- Reported in
- 106 of 122 records (87 %)
- Level when reported
- median 0.18 %, max 1.52 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- oxidation of the free thiol to GSSG — this is the whole stability story
- oxidation is rapid in solution at neutral pH and catalysed by trace metals