MGF impurity profile
Every named species the index has reported alongside MGF, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
1.27%
n=32
Median largest single
0.61%
n=32
Worst single observed
1.15%
n=32
Named species observed
4 / 4
n=4
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| deamidated (+1 Da) | -0.24 | +0.984 | 100% | 0.26 | 1.06 |
| truncated (1-20) | -1.22 | -486.271 | 100% | 0.23 | 1.15 |
| acetyl adduct (+42 Da) | +0.40 | +42.011 | 100% | 0.24 | 0.87 |
| aspartimide (−18 Da) | +0.32 | -18.011 | 97% | 0.29 | 0.61 |
deamidated (+1 Da)
- Retention offset from the main peak
- -0.24 min on RG-2
- Mass difference
- +0.984 Da
- Reported in
- 32 of 32 records (100 %)
- Level when reported
- median 0.26 %, max 1.06 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
truncated (1-20)
- Retention offset from the main peak
- -1.22 min on RG-2
- Mass difference
- -486.271 Da
- Reported in
- 32 of 32 records (100 %)
- Level when reported
- median 0.23 %, max 1.15 %
- Resolution requirement
- Baseline-resolved on the reference method.
acetyl adduct (+42 Da)
- Retention offset from the main peak
- +0.40 min on RG-2
- Mass difference
- +42.011 Da
- Reported in
- 32 of 32 records (100 %)
- Level when reported
- median 0.24 %, max 0.87 %
- Resolution requirement
- Baseline-resolved on the reference method.
aspartimide (−18 Da)
- Retention offset from the main peak
- +0.32 min on RG-2
- Mass difference
- -18.011 Da
- Reported in
- 31 of 32 records (97 %)
- Level when reported
- median 0.29 %, max 0.61 %
- Resolution requirement
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- Asn deamidation
- rapid serum proteolysis (not an assay concern, but the reason PEG-MGF exists)