MOTS-c impurity profile
Every named species the index has reported alongside MOTS-c, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
0.82%
n=112
Median largest single
0.46%
n=112
Worst single observed
1.86%
n=112
Named species observed
4 / 4
n=4
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| des-Met1 (−131 Da) | +0.22 | -131.040 | 96% | 0.14 | 1.49 |
| Met1 sulfoxide (+16 Da) | -0.38 | +15.995 | 94% | 0.19 | 1.86 |
| bis-sulfoxide (+32 Da) | -0.66 | +31.990 | 90% | 0.24 | 1.83 |
| deamidated (+1 Da) | -0.30 | +0.984 | 89% | 0.15 | 1.51 |
des-Met1 (−131 Da)
- Retention offset from the main peak
- +0.22 min on RG-2
- Mass difference
- -131.040 Da
- Reported in
- 107 of 112 records (96 %)
- Level when reported
- median 0.14 %, max 1.49 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
Met1 sulfoxide (+16 Da)
- Retention offset from the main peak
- -0.38 min on RG-2
- Mass difference
- +15.995 Da
- Reported in
- 105 of 112 records (94 %)
- Level when reported
- median 0.19 %, max 1.86 %
- Resolution requirement
- Baseline-resolved on the reference method.
bis-sulfoxide (+32 Da)
- Retention offset from the main peak
- -0.66 min on RG-2
- Mass difference
- +31.990 Da
- Reported in
- 101 of 112 records (90 %)
- Level when reported
- median 0.24 %, max 1.83 %
- Resolution requirement
- Baseline-resolved on the reference method.
deamidated (+1 Da)
- Retention offset from the main peak
- -0.30 min on RG-2
- Mass difference
- +0.984 Da
- Reported in
- 100 of 112 records (89 %)
- Level when reported
- median 0.15 %, max 1.51 %
- Resolution requirement
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- double methionine oxidation — MOTS-c carries two Met residues and both oxidise
- N-terminal Met excision during expression-derived production