NAD+ impurity profile
Every named species the index has reported alongside NAD+, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
1.12%
n=48
Median largest single
0.60%
n=48
Worst single observed
1.21%
n=48
Named species observed
5 / 5
n=5
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| nicotinamide (−541 Da) | -0.68 | -541.052 | 96% | 0.10 | 1.13 |
| nicotinamide mononucleotide (NMN, −331 Da) | -0.42 | -331.058 | 92% | 0.35 | 1.21 |
| ADP-ribose (−121 Da) | -0.26 | -121.040 | 92% | 0.21 | 1.00 |
| NADH (reduced, +2 Da) | +0.34 | +2.016 | 92% | 0.11 | 0.62 |
| α-NAD anomer | +0.18 | mass-identical | 88% | 0.08 | 0.92 |
nicotinamide (−541 Da)
- Retention offset from the main peak
- -0.68 min on RG-2
- Mass difference
- -541.052 Da
- Reported in
- 46 of 48 records (96 %)
- Level when reported
- median 0.10 %, max 1.13 %
- Resolution requirement
- Baseline-resolved on the reference method.
nicotinamide mononucleotide (NMN, −331 Da)
- Retention offset from the main peak
- -0.42 min on RG-2
- Mass difference
- -331.058 Da
- Reported in
- 44 of 48 records (92 %)
- Level when reported
- median 0.35 %, max 1.21 %
- Resolution requirement
- Baseline-resolved on the reference method.
ADP-ribose (−121 Da)
- Retention offset from the main peak
- -0.26 min on RG-2
- Mass difference
- -121.040 Da
- Reported in
- 44 of 48 records (92 %)
- Level when reported
- median 0.21 %, max 1.00 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
NADH (reduced, +2 Da)
- Retention offset from the main peak
- +0.34 min on RG-2
- Mass difference
- +2.016 Da
- Reported in
- 44 of 48 records (92 %)
- Level when reported
- median 0.11 %, max 0.62 %
- Resolution requirement
- Baseline-resolved on the reference method.
α-NAD anomer
- Retention offset from the main peak
- +0.18 min on RG-2
- Mass difference
- mass-identical — chromatography only
- Reported in
- 42 of 48 records (88 %)
- Level when reported
- median 0.08 %, max 0.92 %
- Resolution requirement
- A gradient long enough to separate it. No mass spectrometer will.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- glycosidic hydrolysis to nicotinamide + ADP-ribose, fast above pH 8 and above 30 °C
- anomerisation to the inactive α form