Pramlintide impurity profile
Every named species the index has reported alongside Pramlintide, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
1.16%
n=33
Median largest single
0.57%
n=33
Worst single observed
1.44%
n=33
Named species observed
4 / 4
n=4
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| open-chain (reduced disulfide, +2 Da) | -0.92 | +2.016 | 100% | 0.39 | 1.09 |
| C-terminal free acid (+1 Da) | +0.29 | +0.984 | 100% | 0.19 | 1.21 |
| des-Lys1 (−128 Da) | -0.51 | -128.095 | 94% | 0.14 | 1.44 |
| pramlintide dimer | +2.94 | +3945.091 | 94% | 0.16 | 0.43 |
open-chain (reduced disulfide, +2 Da)
- Retention offset from the main peak
- -0.92 min on RG-2
- Mass difference
- +2.016 Da
- Reported in
- 33 of 33 records (100 %)
- Level when reported
- median 0.39 %, max 1.09 %
- Resolution requirement
- Baseline-resolved on the reference method.
C-terminal free acid (+1 Da)
- Retention offset from the main peak
- +0.29 min on RG-2
- Mass difference
- +0.984 Da
- Reported in
- 33 of 33 records (100 %)
- Level when reported
- median 0.19 %, max 1.21 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
des-Lys1 (−128 Da)
- Retention offset from the main peak
- -0.51 min on RG-2
- Mass difference
- -128.095 Da
- Reported in
- 31 of 33 records (94 %)
- Level when reported
- median 0.14 %, max 1.44 %
- Resolution requirement
- Baseline-resolved on the reference method.
pramlintide dimer
- Retention offset from the main peak
- +2.94 min on RG-2
- Mass difference
- +3945.091 Da
- Reported in
- 31 of 33 records (94 %)
- Level when reported
- median 0.16 %, max 0.43 %
- Resolution requirement
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- disulfide reduction and subsequent scrambling
- C-terminal amide hydrolysis below pH 3