AssayIndex
Cross-section

Thymulin impurity profile

Every named species the index has reported alongside Thymulin, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.

18 recordsmedian 99.06 %spec 98.0 %
Median total impurities 0.94% n=18
Median largest single 0.61% n=18
Worst single observed 0.99% n=18
Named species observed 4 / 4 n=4
Named speciesΔRT (min)Δ mass (Da)Seen inMedian % areaMax % area
des-Ser truncate (−87 Da)-0.28-87.032100%0.470.86
deamidated (+1 Da)-0.18+0.984100%0.080.99
zinc-free apo-thymulin-0.40-63.929100%0.050.28
diketopiperazine (−18 Da)+0.22-18.011100%0.150.66

des-Ser truncate (−87 Da)

Retention offset from the main peak
-0.28 min on RG-2
Mass difference
-87.032 Da
Reported in
18 of 18 records (100 %)
Level when reported
median 0.47 %, max 0.86 %
Resolution requirement
Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.

deamidated (+1 Da)

Retention offset from the main peak
-0.18 min on RG-2
Mass difference
+0.984 Da
Reported in
18 of 18 records (100 %)
Level when reported
median 0.08 %, max 0.99 %
Resolution requirement
Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.

zinc-free apo-thymulin

Retention offset from the main peak
-0.40 min on RG-2
Mass difference
-63.929 Da
Reported in
18 of 18 records (100 %)
Level when reported
median 0.05 %, max 0.28 %
Resolution requirement
Baseline-resolved on the reference method.

diketopiperazine (−18 Da)

Retention offset from the main peak
+0.22 min on RG-2
Mass difference
-18.011 Da
Reported in
18 of 18 records (100 %)
Level when reported
median 0.15 %, max 0.66 %
Resolution requirement
Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.

Degradation pathways

Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.

  • zinc dissociation
  • Asn deamidation