AssayIndex

AX-2026-042130

Semax · 10 mg declared · Hunan Aslsen Technology Co., Ltd. · lot HOMO-2602-S8315

MARGINALVERIFIED
Report ID
AX-2026-042130
Compound
Semax
Compound class
Neuroactive and cognitive peptides
Supplier
Homopeptide — Hunan Aslsen Technology Co., Ltd.
Batch / lot
HOMO-2602-S8315
Declared label claim
10mg
Laboratory
Janoshik Analytical
Assay date
09 Feb 2026
Submitted
26 Feb 2026
Quarter
2026-Q1
Submitter
g.balogun
Sample provenance
Laboratory-retained
Section 1

Results

Measured against the index acceptance specification for the neuroactive and cognitive peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

ParameterMethodResultUnitIndex spec
PurityRP-HPLC, 30 min gradient, 214 nm99.56% area≥ 98.5PASS
Total impuritiesRP-HPLC, sum of reported peaks0.44% area≤ 1.5PASS
Largest single impurityRP-HPLC — N-acetyl semax (+42 Da)0.27% area≤ 1.00PASS
Identity — theoretical monoisotopic masscalculated from molecular formula813.3541Da—NOT TESTED
Identity — observed monoisotopic massLC-MS, high resolution (ESI-TOF/QTOF)813.3557Da—NOT TESTED
Mass accuracyobserved vs theoretical+2.0ppm± 15PASS
Total peptide contentby difference from the gravimetric mass balance84.75%w/w—NOT TESTED
Target-compound contenttotal peptide content × purity84.38%w/w—NOT TESTED
Gross fill (as weighed)five-place balance, under nitrogen12.25mg10 mg declaredNOT TESTED
Recovered target peptidegross fill × target content10.337mg10 mg claimNOT TESTED
Recovery against label claimrecovered ÷ claim103.4%90.0 – 110.0PASS
Water contentKarl Fischer, coulometric, duplicate9.36%≤ 12.0PASS
Counter-ion (acetate)anion-exchange ion chromatography5.39%≤ 10.0PASS
Residual solvent + inorganic residueestimated — see /methods/residual-solvents-q3c/0.50%—NOT TESTED
Endotoxinkinetic chromogenic LAL0.40EU/mg≤ 5.0PASS
Sterilitymembrane filtration, USP <71>, 14 dNo growth at 14 d—no growthPASS
Appearancevisual, diffuse light, black and white backgroundOff-white lyophilised cake, intact—white to off-white intact cakePASS
Reconstitution clarityreconstituted per submitter procedureClear, colourless, no visible particulates—clear, no visible particulatesPASS
pH (reconstituted, 1 mg/mL)potentiometric, two-point calibration5.14—2.5 – 8.0PASS
Retention time (observed)RP-HPLC, 30 min gradient7.20min7.06 ± 0.25 (normalised)PASS
Peak asymmetry (10 % height)Ph. Eur. 2.2.460.99—0.80 – 1.50PASS
Plate count, main peakN = 5.54 (t/w½)²111,312—≥ 20,000PASS
Resolution to nearest impurityvs diketopiperazine (−18 Da)1.81—no index criterionREPORTED
Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

05367107341610121469 Semax7.20 min · 99.56 % 7.62 same trace · ×200 vertical expansion, baseline-corrected · main peak off-scale reporting threshold 0.05 % area 05101520253035 Retention time (min) Response (mAU)
Figure 1. RP-HPLC trace, 30 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 111,312, and skew follows the reported asymmetry factor of 0.99. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.27 % impurity against a 99.56 % main peak is a line two pixels tall; the main peak runs off-scale there by design.
#PeakRTArea (µV·s)% areaΔ mass (Da)
1Semax — main peak7.204,838,92599.56—
2N-acetyl semax (+42 Da)7.6213,1230.27+42.011
3diketopiperazine (−18 Da)7.365,3460.11-18.011
4des-Met (−131 Da)7.401,9440.04-131.040
5Met1 sulfoxide (+16 Da)6.849720.02+15.995
Closure check — purity + Σ impurities 100.00CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 813.3541 Da, computed from C37H51N9O10S. All ppm figures on this site are against monoisotopic values — see why that matters.

0255075100350450550650750850 [M+1H]1+814.36[M+2H]2+407.69 m/z Relative abundance (%)
Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 813.3557 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.
ChargeTheoretical m/zObserved m/zΔ m/zRel. abundance (%)
2+407.6843407.68510.000854.1
1+814.3614814.36300.0016100.0
Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

ComponentDetermination%w/w of cake
WaterKarl Fischer9.36
Counter-ion (acetate)ion chromatography5.39
Residual solvent + inorganic residueestimated0.50
Total peptide contentby difference84.75
Total100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

FigureSupplier COAThis reportIndex note
Purity (% area)100.099.56divergence +0.26 pp vs batch median
Total impurities (% area)-0.840.44
Largest single impurity (% area)not stated0.27
Water (%)7.39.36
Counter-ion (%)not stated5.39
Peptide content (%w/w)87.984.75
Endotoxin (EU/mg)not stated0.40
Method stated on certificateYES—column, gradient and wavelength given
Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance
Laboratory-retained
Evidentiary weight
Provenance documented, selection uncontrolled.
Chain of custody
Laboratory retained sample (−20 °C, 24 d since original receipt) → re-assay.
Assay date
09 Feb 2026
Submitted to the index
26 Feb 2026
Laboratory
Janoshik Analytical, Ostrava, Czechia
Method basis
30 min gradient · high-resolution MS
Verification status
VERIFIED
Section 6

Annotations

No annotations on this record. Annotations are short signed technical comments from index contributors; see how to contribute.

Section 7

Related records

Same batch — lot HOMO-2602-S8315 n=12

ReportLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-042124HOMO-2602-S8315Janoshik2026-Q199.849.15103.2Supplier-supplied2MARGINAL
AX-2026-042125HOMO-2602-S8315VendorInvestigate2026-Q199.318.81—Buyer-sourced3MARGINAL
AX-2026-042126HOMO-2602-S8315VendorInvestigate2026-Q199.178.54—Buyer-sourced3MARGINAL
AX-2026-042127HOMO-2602-S8315Janoshik2026-Q199.239.9198.3Supplier-supplied2MARGINAL
AX-2026-042128HOMO-2602-S8315Janoshik2026-Q199.939.16105.2Supplier-supplied2MARGINAL
AX-2026-042129HOMO-2602-S8315PeptideMeter2026-Q199.628.83—Supplier-supplied2MARGINAL
AX-2026-042131HOMO-2602-S8315Kestrel2026-Q199.939.49101.5Supplier-supplied3MARGINAL
AX-2026-042132HOMO-2602-S8315Janoshik2026-Q199.939.37106.8Supplier-supplied3MARGINAL
AX-2026-042133HOMO-2602-S8315Janoshik2026-Q199.939.29—Supplier-supplied2MARGINAL
AX-2026-042134HOMO-2602-S8315Janoshik2026-Q199.748.45—Supplier-supplied2MARGINAL
AX-2026-042135HOMO-2602-S8315Janoshik2026-Q199.749.3795.1Supplier-supplied2MARGINAL

Cross-sections

Other recent Semax records in the index.
ReportSupplierLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-042368HJHJ-2603-V4363Nordanalyt2026-Q399.177.26106.3Supplier-supplied1MARGINAL
AX-2026-042367HJHJ-2603-V4363Kestrel2026-Q399.106.7893.9Supplier-supplied1MARGINAL
AX-2026-042260HomopeptideHOMO-2605-S1342Medutest2026-Q399.446.0692.4Laboratory-retained1FAIL
AX-2026-042370HJHJ-2603-V4363Janoshik2026-Q398.947.16104.0Supplier-supplied1MARGINAL
AX-2026-042369HJHJ-2603-V4363Janoshik2026-Q399.267.6795.0Supplier-supplied1MARGINAL
AX-2026-041947TFCTFC-2602-F4591Nordanalyt2026-Q299.298.9892.3Supplier-supplied0PASS
AX-2026-041940TFCTFC-2602-F4591Janoshik2026-Q299.068.3898.8Buyer-sourced0PASS
AX-2026-043141SSASSA-2604-L7628Kestrel2026-Q298.848.5893.2Supplier-supplied2MARGINAL
Other recent Homopeptide records in the index.
ReportCompoundLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-042181IpamorelinHOMO-2608-A9045Janoshik2026-Q399.784.79—Supplier-supplied1MARGINAL
AX-2026-042172SemaglutideHOMO-2604-X8921Janoshik2026-Q399.645.1691.9Supplier-supplied1MARGINAL
AX-2026-042278SLU-PP-332HOMO-2602-Y2822Kestrel2026-Q399.714.42—Supplier-supplied0PASS
AX-2026-042180IpamorelinHOMO-2608-A9045Janoshik2026-Q399.394.74105.5Supplier-supplied1MARGINAL
AX-2026-042184IpamorelinHOMO-2608-A9045Kestrel2026-Q399.274.79103.2Supplier-supplied1MARGINAL
AX-2026-042274SLU-PP-332HOMO-2602-Y2822VendorInvestigate2026-Q398.714.72—Laboratory-retained0PASS
AX-2026-042183IpamorelinHOMO-2608-A9045VendorInvestigate2026-Q399.384.97—Buyer-sourced1MARGINAL
AX-2026-042194RetatrutideHOMO-2605-V9833PeptideMeter2026-Q398.904.0798.8Supplier-supplied3MARGINAL