Kisspeptin-10 impurity profile
Every named species the index has reported alongside Kisspeptin-10, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
0.73%
n=70
Median largest single
0.41%
n=70
Worst single observed
1.29%
n=70
Named species observed
4 / 4
n=4
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| des-Tyr (−163 Da) | -0.56 | -163.063 | 93% | 0.16 | 0.69 |
| C-terminal free acid (+1 Da) | +0.18 | +0.984 | 93% | 0.13 | 0.46 |
| oxidised Trp (+16 Da) | -0.34 | +15.995 | 86% | 0.24 | 1.29 |
| [D-Trp]-epimer | -0.12 | mass-identical | 84% | 0.18 | 1.15 |
des-Tyr (−163 Da)
- Retention offset from the main peak
- -0.56 min on RG-2
- Mass difference
- -163.063 Da
- Reported in
- 65 of 70 records (93 %)
- Level when reported
- median 0.16 %, max 0.69 %
- Resolution requirement
- Baseline-resolved on the reference method.
C-terminal free acid (+1 Da)
- Retention offset from the main peak
- +0.18 min on RG-2
- Mass difference
- +0.984 Da
- Reported in
- 65 of 70 records (93 %)
- Level when reported
- median 0.13 %, max 0.46 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
oxidised Trp (+16 Da)
- Retention offset from the main peak
- -0.34 min on RG-2
- Mass difference
- +15.995 Da
- Reported in
- 60 of 70 records (86 %)
- Level when reported
- median 0.24 %, max 1.29 %
- Resolution requirement
- Baseline-resolved on the reference method.
[D-Trp]-epimer
- Retention offset from the main peak
- -0.12 min on RG-2
- Mass difference
- mass-identical — chromatography only
- Reported in
- 59 of 70 records (84 %)
- Level when reported
- median 0.18 %, max 1.15 %
- Resolution requirement
- A gradient long enough to separate it. No mass spectrometer will.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- Trp oxidation
- C-terminal amide hydrolysis — the amide is required for activity