AX-2025-020538

MOTS-c · 10 mg declared · Shanghai Sigma-Audley New Material Technology Co., Ltd. · lot SSA-2501-M6043

FAILPURITY ONLY

Report ID: AX-2025-020538
Compound: MOTS-c
Compound class: Metabolic and mitochondrial peptides
Supplier: SSA — Shanghai Sigma-Audley New Material Technology Co., Ltd.
Batch / lot: SSA-2501-M6043
Declared label claim: 10mg
Laboratory: PeptideMeter
Assay date: 23 Jan 2025
Submitted: 25 Jan 2025
Quarter: 2025-Q1
Submitter: ha.hartmann
Sample provenance: Buyer-sourced

Section 1

Results

Measured against the index acceptance specification for the metabolic and mitochondrial peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 28 min gradient, 214 nm	97.96	% area	≥ 98.0	FAIL
Total impurities	RP-HPLC, sum of reported peaks	2.04	% area	≤ 2.0	FAIL
Largest single impurity	RP-HPLC — bis-sulfoxide (+32 Da)	1.40	% area	≤ 1.00	FAIL
Identity	not ordered on this submission	not determined	—	required for VERIFIED status	NOT TESTED
Total peptide content	by difference from the gravimetric mass balance	90.89	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	89.04	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	10.02	mg	10 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	8.922	mg	10 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	89.2	%	90.0 – 110.0	FAIL
Water content	Karl Fischer, coulometric, duplicate	2.82	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	5.76	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.53	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	Off-white lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear with a single visible fibre	—	clear, no visible particulates	FAIL
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	5.25	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 28 min gradient	9.73	min	9.78 ± 0.30 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.20	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	54,023	—	≥ 20,000	PASS
Resolution to nearest impurity	vs des-Met1 (−131 Da)	1.20	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 28 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 54,023, and skew follows the reported asymmetry factor of 1.20. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 1.40 % impurity against a 97.96 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	MOTS-c — main peak	9.73	4,761,160	97.96	—
2	bis-sulfoxide (+32 Da)	9.11	68,044	1.40	+31.990
3	deamidated (+1 Da)	9.45	12,637	0.26	+0.984
4	Met1 sulfoxide (+16 Da)	9.38	9,721	0.20	+15.995
5	des-Met1 (−131 Da)	9.94	8,749	0.18	-131.040
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	2.82
Counter-ion (acetate)	ion chromatography	5.76
Residual solvent + inorganic residue	estimated	0.53
Total peptide content	by difference	90.89
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	97.90	97.96	divergence -0.03 pp vs batch median
Total impurities (% area)	not stated	2.04
Largest single impurity (% area)	not stated	1.40
Water (%)	1.5	2.82
Counter-ion (%)	not stated	5.76
Peptide content (%w/w)	93.7	90.89
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (−20 °C, 16 d) → laboratory (courier, dry ice, 6 d) → assay.
Assay date: 23 Jan 2025
Submitted to the index: 25 Jan 2025
Laboratory: PeptideMeter, Tallinn, Estonia
Method basis: 28 min gradient · high-resolution MS
Verification status: PURITY ONLY

What this report does not establish

That the compound is the compound named. No identity confirmation was ordered on this submission. A purity figure describes chromatographic homogeneity of whatever eluted; it does not name it.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations

No annotations on this record. Annotations are short signed technical comments from index contributors; see how to contribute.

Section 7

Related records

Same batch — lot SSA-2501-M6043 n=14

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2025-020537	SSA-2501-M6043	Janoshik	2025-Q1	98.21	2.90	—	Supplier-supplied	0	PASS
AX-2025-020539	SSA-2501-M6043	Medutest	2025-Q1	99.11	2.42	92.5	Supplier-supplied	0	PASS
AX-2025-020540	SSA-2501-M6043	Kestrel	2025-Q1	98.78	2.48	104.9	Supplier-supplied	0	PASS
AX-2025-020541	SSA-2501-M6043	Nordanalyt	2025-Q1	98.07	3.64	105.8	Supplier-supplied	0	PASS
AX-2025-020542	SSA-2501-M6043	Medutest	2025-Q1	97.77	2.19	94.0	Supplier-supplied	0	FAIL
AX-2025-020543	SSA-2501-M6043	Medutest	2025-Q1	98.44	3.49	—	Supplier-supplied	2	MARGINAL
AX-2025-020544	SSA-2501-M6043	Janoshik	2025-Q1	97.34	2.85	88.9	Laboratory-retained	1	FAIL
AX-2025-020545	SSA-2501-M6043	Janoshik	2025-Q1	97.89	2.19	104.0	Supplier-supplied	0	FAIL
AX-2025-020546	SSA-2501-M6043	Janoshik	2025-Q1	98.45	2.73	89.1	Supplier-supplied	1	MARGINAL
AX-2025-020547	SSA-2501-M6043	PeptideMeter	2025-Q1	97.76	2.58	102.2	Supplier-supplied	0	FAIL
AX-2025-020548	SSA-2501-M6043	VendorInvestigate	2025-Q1	97.46	2.21	90.6	Buyer-sourced	0	FAIL
AX-2025-020549	SSA-2501-M6043	Janoshik	2025-Q1	97.80	3.18	—	Buyer-sourced	0	FAIL
AX-2025-020550	SSA-2501-M6043	Nordanalyt	2025-Q1	97.89	3.12	—	Laboratory-retained	0	FAIL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent MOTS-c records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041952	TFC	TFC-2605-R4770	PeptideMeter	2026-Q2	99.65	5.60	95.5	Supplier-supplied	0	PASS
AX-2026-043416	WXT	WXT-2511-P4848	Janoshik	2026-Q2	98.23	5.14	106.7	Supplier-supplied	0	PASS
AX-2026-043149	SSA	SSA-2511-W7415	Janoshik	2026-Q2	98.00	5.42	—	Supplier-supplied	0	PASS
AX-2026-043498	BCH	BCH-2511-L5815	Janoshik	2026-Q2	96.96	3.75	93.4	Supplier-supplied	0	FAIL
AX-2026-043150	SSA	SSA-2511-W7415	Janoshik	2026-Q2	98.33	5.76	—	Supplier-supplied	0	PASS
AX-2026-043152	SSA	SSA-2511-W7415	Janoshik	2026-Q2	98.64	5.48	88.9	Supplier-supplied	2	MARGINAL
AX-2026-041953	TFC	TFC-2605-R4770	Janoshik	2026-Q2	99.72	5.27	90.8	Supplier-supplied	2	FAIL
AX-2026-043153	SSA	SSA-2511-W7415	Medutest	2026-Q2	98.78	5.52	—	Supplier-supplied	0	PASS

Other recent SSA records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043144	GHK-Cu	SSA-2604-V7620	Janoshik	2026-Q2	98.35	5.25	105.8	Supplier-supplied	0	PASS
AX-2026-043141	Semax	SSA-2604-L7628	Kestrel	2026-Q2	98.84	8.58	93.2	Supplier-supplied	2	MARGINAL
AX-2026-043117	Mazdutide	SSA-2605-G6781	Janoshik	2026-Q2	99.23	3.67	90.4	Supplier-supplied	1	MARGINAL
AX-2026-043136	BPC-157	SSA-2601-N6752	Janoshik	2026-Q2	97.75	4.32	—	Supplier-supplied	2	FAIL
AX-2026-043118	Mazdutide	SSA-2605-G6781	Medutest	2026-Q2	98.29	2.65	88.7	Buyer-sourced	2	MARGINAL
AX-2026-043138	BPC-157	SSA-2601-N6752	Janoshik	2026-Q2	98.00	4.30	101.0	Supplier-supplied	1	MARGINAL
AX-2026-043137	BPC-157	SSA-2601-N6752	Janoshik	2026-Q2	97.41	4.36	104.5	Laboratory-retained	1	FAIL
AX-2026-043146	Enclomiphene citrate	SSA-2603-C6950	Medutest	2026-Q2	97.92	3.11	92.9	Buyer-sourced	2	FAIL