AX-2025-021020 · 5-Amino-1MQ · WXT lot WXT-2505-M3910

Section 1

Results

Measured against the index acceptance specification for the non-peptide adjuncts class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 35 min gradient, 214 nm	98.58	% area	≥ 98.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	1.42	% area	≤ 2.0	PASS
Largest single impurity	RP-HPLC — 5-nitro-1MQ (unreduced precursor, +30 Da)	0.90	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	285.9967	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, high resolution (ESI-TOF/QTOF)	285.9962	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	-1.9	ppm	± 10	PASS
Peptide content	not ordered on this submission	not determined	—	required to check label claim	NOT TESTED
Water content	Karl Fischer, coulometric, duplicate	2.88	%	≤ 10.0	PASS
Counter-ion (iodide)	anion-exchange ion chromatography	46.22	%	≤ 12.0	FAIL
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.37	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	0.27	EU/mg	≤ 5.0	PASS
Sterility	membrane filtration, USP <71>, 14 d	No growth at 14 d	—	no growth	PASS
Appearance	visual, diffuse light, black and white background	White cake, partially collapsed	—	white to off-white intact cake	FAIL
Reconstitution clarity	reconstituted per submitter procedure	Clear after 90 s gentle swirling, no particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	5.35	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 35 min gradient	6.24	min	6.25 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	0.97	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	54,862	—	≥ 20,000	PASS
Resolution to nearest impurity	vs free iodide (counter-ion, by IC)	0.00	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 35 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 54,862, and skew follows the reported asymmetry factor of 0.97. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.90 % impurity against a 98.58 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	5-Amino-1MQ — main peak	6.24	4,791,294	98.58	—
2	5-nitro-1MQ (unreduced precursor, +30 Da)	7.03	43,743	0.90	+29.974
3	1-methylquinolinium (des-amino, −15 Da)	5.87	11,665	0.24	-15.011
4	8-amino regioisomer	6.50	9,235	0.19	0.000
5	free iodide (counter-ion, by IC)	6.24	4,374	0.09	0.000
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 285.9967 Da, computed from C10H11N2+ · I−. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 285.9962 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
1+	287.0040	287.0035	-0.0005	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	98.65	98.58	divergence +0.14 pp vs batch median
Total impurities (% area)	1.35	1.42
Largest single impurity (% area)	not stated	0.90
Water (%)	1.4	2.88
Counter-ion (%)	not stated	46.22
Peptide content (%w/w)	not stated	not determined
Endotoxin (EU/mg)	not stated	0.27
Method stated on certificate	NO	—	no method section — the result cannot be reconciled against any other

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Supplier-supplied
Evidentiary weight: Weaker evidence. Read with the caveat below.
Chain of custody: Supplier despatch → laboratory direct (courier, cold pack, 4 d) → assay. Submitter did not handle the vial.
Assay date: 18 Jun 2025
Submitted to the index: 05 Jul 2025
Laboratory: Nordanalyt Laboratories, Uppsala, Sweden
Method basis: 35 min gradient · high-resolution MS
Verification status: VERIFIED

What this report does not establish

How much of the named compound is in the vial. Peptide content was not determined, so recovered mass cannot be calculated and the label claim cannot be checked.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations

No annotations on this record. Annotations are short signed technical comments from index contributors; see how to contribute.

Section 7

Related records

Same batch — lot WXT-2505-M3910 n=10

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2025-021014	WXT-2505-M3910	VendorInvestigate	2025-Q2	97.95	3.13	105.5	Buyer-sourced	1	FAIL
AX-2025-021015	WXT-2505-M3910	Janoshik	2025-Q2	98.61	2.77	—	Supplier-supplied	1	FAIL
AX-2025-021016	WXT-2505-M3910	Janoshik	2025-Q2	98.43	2.83	—	Buyer-sourced	1	FAIL
AX-2025-021017	WXT-2505-M3910	Medutest	2025-Q2	98.61	2.51	—	Supplier-supplied	1	FAIL
AX-2025-021018	WXT-2505-M3910	Medutest	2025-Q2	98.29	2.58	—	Buyer-sourced	1	FAIL
AX-2025-021019	WXT-2505-M3910	Janoshik	2025-Q2	98.72	2.74	—	Supplier-supplied	1	FAIL
AX-2025-021021	WXT-2505-M3910	Janoshik	2025-Q2	98.05	2.95	90.6	Buyer-sourced	2	FAIL
AX-2025-021022	WXT-2505-M3910	Janoshik	2025-Q2	97.99	2.71	100.3	Laboratory-retained	1	FAIL
AX-2025-021023	WXT-2505-M3910	Kestrel	2025-Q2	98.82	2.93	107.5	Supplier-supplied	1	FAIL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent 5-Amino-1MQ records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042753	GGPeps	GGPE-2512-M9695	Medutest	2026-Q2	99.21	4.61	93.5	Supplier-supplied	2	FAIL
AX-2026-042970	KP	KP-2511-W4792	Janoshik	2026-Q2	98.89	4.03	91.0	Supplier-supplied	2	FAIL
AX-2026-042969	KP	KP-2511-W4792	Janoshik	2026-Q2	99.33	3.94	—	Buyer-sourced	2	FAIL
AX-2026-042967	KP	KP-2511-W4792	Janoshik	2026-Q2	99.49	4.14	—	Supplier-supplied	2	FAIL
AX-2026-042754	GGPeps	GGPE-2512-M9695	PeptideMeter	2026-Q2	99.20	4.90	96.8	Supplier-supplied	2	FAIL
AX-2026-042971	KP	KP-2511-W4792	Janoshik	2026-Q2	99.45	4.19	107.1	Supplier-supplied	2	FAIL
AX-2026-042968	KP	KP-2511-W4792	Janoshik	2026-Q2	98.87	4.32	91.5	Buyer-sourced	2	FAIL
AX-2026-042012	TFC	TFC-2508-L6321	Janoshik	2026-Q1	99.93	5.84	95.7	Supplier-supplied	0	FAIL

Other recent WXT records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043488	Pramlintide	WXT-2603-D6052	Janoshik	2026-Q3	97.87	4.37	—	Supplier-supplied	1	MARGINAL
AX-2026-043406	Semaglutide	WXT-2604-C4728	Janoshik	2026-Q3	97.24	4.65	—	Supplier-supplied	1	MARGINAL
AX-2026-043421	Sermorelin	WXT-2608-P4867	Janoshik	2026-Q3	98.15	3.09	—	Supplier-supplied	0	PASS
AX-2026-043420	Sermorelin	WXT-2608-P4867	Medutest	2026-Q3	98.62	3.07	96.4	Supplier-supplied	0	PASS
AX-2026-043423	Sermorelin	WXT-2608-P4867	Janoshik	2026-Q3	98.28	3.86	98.5	Buyer-sourced	0	PASS
AX-2026-043401	Semaglutide	WXT-2604-C4728	Medutest	2026-Q3	97.70	4.78	91.5	Supplier-supplied	1	MARGINAL
AX-2026-043400	Semaglutide	WXT-2604-C4728	Nordanalyt	2026-Q3	96.86	4.11	106.4	Laboratory-retained	1	FAIL
AX-2026-043422	Sermorelin	WXT-2608-P4867	Medutest	2026-Q3	98.35	3.59	—	Supplier-supplied	1	MARGINAL