AX-2025-019850 · AOD-9604 · SWB lot SWB-2502-N4413

Section 1

Results

Measured against the index acceptance specification for the metabolic and mitochondrial peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 25 min gradient, 214 nm	99.18	% area	≥ 98.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.82	% area	≤ 2.0	PASS
Largest single impurity	RP-HPLC — AOD dimer	0.52	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	1815.8672	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, unit resolution (single quadrupole)	1815.8518	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	-8.5	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	87.45	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	86.73	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	2.31	mg	2 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	2.003	mg	2 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	100.2	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	5.44	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	6.83	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.28	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White powder, no defined cake (melt-back)	—	white to off-white intact cake	FAIL
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	4.52	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 25 min gradient	9.58	min	9.55 ± 0.30 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	0.92	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	45,731	—	≥ 20,000	PASS
Resolution to nearest impurity	vs Met sulfoxide (+16 Da)	2.00	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 25 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 45,731, and skew follows the reported asymmetry factor of 0.92. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.52 % impurity against a 99.18 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	AOD-9604 — main peak	9.58	4,820,455	99.18	—
2	AOD dimer	11.33	25,274	0.52	+1813.852
3	open-chain (reduced disulfide, +2 Da)	9.07	8,263	0.17	+2.016
4	des-Tyr AOD (−163 Da)	8.88	3,888	0.08	-163.063
5	Met sulfoxide (+16 Da)	9.21	2,430	0.05	+15.995
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 1815.8672 Da, computed from C78H123N23O23S2. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, unit-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 1815.8518 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
4+	454.9741	454.9702	-0.0039	8.6
3+	606.2963	606.2912	-0.0051	54.3
2+	908.9409	908.9332	-0.0077	100.0
1+	1816.8745	1816.8591	-0.0154	54.3

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	5.44
Counter-ion (acetate)	ion chromatography	6.83
Residual solvent + inorganic residue	estimated	0.28
Total peptide content	by difference	87.45
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.64	99.18	divergence +0.48 pp vs batch median
Total impurities (% area)	0.36	0.82
Largest single impurity (% area)	not stated	0.52
Water (%)	3.2	5.44
Counter-ion (%)	not stated	6.83
Peptide content (%w/w)	not stated	87.45
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → third-party forwarder (17 d) → laboratory (courier, cold pack, 9 d) → assay.
Assay date: 03 Feb 2025
Submitted to the index: 07 Feb 2025
Laboratory: Medutest, Ljubljana, Slovenia
Method basis: 25 min gradient · unit-resolution MS
Verification status: IDENTITY CONSISTENT

What this report does not establish

That the compound is not a near-isobaric alternative. Identity was confirmed at unit mass resolution, which cannot exclude species differing by 1 or 2 Da. The specific alternatives not excluded are named in the identity block above.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations

No annotations on this record. Annotations are short signed technical comments from index contributors; see how to contribute.

Section 7

Related records

Same batch — lot SWB-2502-N4413 n=6

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2025-019847	SWB-2502-N4413	Janoshik	2025-Q1	99.19	5.19	—	Supplier-supplied	PASS
AX-2025-019848	SWB-2502-N4413	Janoshik	2025-Q1	99.60	4.96	—	Supplier-supplied	PASS
AX-2025-019849	SWB-2502-N4413	Janoshik	2025-Q1	99.01	4.29	—	Supplier-supplied	PASS
AX-2025-019851	SWB-2502-N4413	PeptideMeter	2025-Q1	98.77	4.87	97.2	Supplier-supplied	PASS
AX-2025-019852	SWB-2502-N4413	Medutest	2025-Q1	99.14	5.60	—	Supplier-supplied	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent AOD-9604 records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042300	HJ	HJ-2601-N2483	Janoshik	2026-Q2	98.77	5.32	—	Buyer-sourced	1	MARGINAL
AX-2026-042297	HJ	HJ-2601-N2483	Medutest	2026-Q2	99.00	4.72	99.5	Supplier-supplied	1	MARGINAL
AX-2026-042299	HJ	HJ-2601-N2483	VendorInvestigate	2026-Q2	98.14	5.21	103.7	Buyer-sourced	1	MARGINAL
AX-2026-042296	HJ	HJ-2601-N2483	Janoshik	2026-Q2	98.85	5.76	97.1	Supplier-supplied	1	MARGINAL
AX-2026-042301	HJ	HJ-2601-N2483	VendorInvestigate	2026-Q2	98.60	5.36	97.9	Buyer-sourced	1	MARGINAL
AX-2026-042298	HJ	HJ-2601-N2483	Janoshik	2026-Q2	99.02	5.14	96.5	Supplier-supplied	1	MARGINAL
AX-2026-042295	HJ	HJ-2601-N2483	Medutest	2026-Q2	98.88	5.80	93.8	Supplier-supplied	1	MARGINAL
AX-2026-041474	QYB	QYB-2508-E6017	Janoshik	2026-Q1	99.62	3.75	—	Supplier-supplied	0	PASS

Other recent SWB records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042461	Kisspeptin-10	SWB-2603-D6238	Janoshik	2026-Q3	98.35	4.65	96.5	Supplier-supplied	3	FAIL
AX-2026-042479	Melanotan II	SWB-2603-A5707	Janoshik	2026-Q3	99.16	3.67	94.5	Supplier-supplied	1	MARGINAL
AX-2026-042481	Melanotan II	SWB-2603-A5707	Medutest	2026-Q3	98.95	3.37	90.8	Supplier-supplied	1	MARGINAL
AX-2026-042460	Kisspeptin-10	SWB-2603-D6238	Kestrel	2026-Q3	99.55	4.21	90.8	Supplier-supplied	3	MARGINAL
AX-2026-042483	Melanotan II	SWB-2603-A5707	Janoshik	2026-Q3	99.38	3.73	107.2	Supplier-supplied	1	MARGINAL
AX-2026-042398	Semaglutide	SWB-2602-X5278	Nordanalyt	2026-Q3	99.08	4.48	—	Supplier-supplied	1	MARGINAL
AX-2026-042399	Semaglutide	SWB-2602-X5278	Janoshik	2026-Q3	98.56	3.82	—	Supplier-supplied	1	MARGINAL
AX-2026-042499	BPC-157	SWB-2607-Y5787	Kestrel	2026-Q3	99.93	4.25	94.4	Supplier-supplied	0	PASS