AX-2026-043732 · N-Acetyl Semax Amide · BCH lot BCH-2602-E8475

Section 1

Results

Measured against the index acceptance specification for the neuroactive and cognitive peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 25 min gradient, 214 nm	97.80	% area	≥ 98.5	FAIL
Total impurities	RP-HPLC, sum of reported peaks	2.20	% area	≤ 1.5	FAIL
Largest single impurity	RP-HPLC — Met sulfoxide (+16 Da)	0.93	% area	≤ 1.00	PASS
Identity	not ordered on this submission	not determined	—	required for VERIFIED status	NOT TESTED
Peptide content	not ordered on this submission	not determined	—	required to check label claim	NOT TESTED
Water content	Karl Fischer, coulometric, duplicate	8.90	%	≤ 12.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	6.01	%	≤ 10.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.45	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	Off-white lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear after 90 s gentle swirling, no particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	5.23	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 25 min gradient	6.40	min	6.45 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.14	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	49,330	—	≥ 20,000	PASS
Resolution to nearest impurity	vs N-acetyl semax free acid (+1 Da)	1.55	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 25 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 49,330, and skew follows the reported asymmetry factor of 1.14. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.93 % impurity against a 97.80 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	N-Acetyl Semax Amide — main peak	6.40	4,753,383	97.80	—
2	Met sulfoxide (+16 Da)	6.12	45,201	0.93	+15.995
3	N-acetyl semax free acid (+1 Da)	6.58	30,134	0.62	+0.984
4	semax (des-acetyl, des-amide, −41 Da)	5.93	16,039	0.33	-41.027
5	des-acetyl amide (−42 Da)	6.07	15,553	0.32	-42.011
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	97.80	97.80	divergence -0.05 pp vs batch median
Total impurities (% area)	2.20	2.20
Largest single impurity (% area)	0.91	0.93
Water (%)	not stated	8.90
Counter-ion (%)	not stated	6.01
Peptide content (%w/w)	not stated	not determined
Endotoxin (EU/mg)	1.62	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (ambient, 20 d) → laboratory (courier, cold pack, 8 d) → assay.
Assay date: 12 Jul 2026
Submitted to the index: 18 Jul 2026
Laboratory: Medutest, Ljubljana, Slovenia
Method basis: 25 min gradient · unit-resolution MS
Verification status: PURITY ONLY

What this report does not establish

That the compound is the compound named. No identity confirmation was ordered on this submission. A purity figure describes chromatographic homogeneity of whatever eluted; it does not name it.
How much of the named compound is in the vial. Peptide content was not determined, so recovered mass cannot be calculated and the label claim cannot be checked.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 3

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

mass_defect18 Jul 2026

Before assigning Met sulfoxide (+16 Da) from retention alone, check the mass defect on the impurity peak. Plausible and implausible assignments separate cleanly and the check costs nothing.

uncertainty_budget18 Jul 2026

The gap between 97.80 % and the class specification should be read against an expanded uncertainty of roughly ±0.5 pp. A shortfall inside that band is not a finding; a shortfall well outside it is.

endotoxin_limit18 Jul 2026

Sterility and endotoxin are independent properties and are constantly confused. A sterile-filtered lot can carry a high burden and frequently does.

Section 7

Related records

Same batch — lot BCH-2602-E8475 n=10

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043726	BCH-2602-E8475	Janoshik	2026-Q3	97.89	9.67	104.2	Buyer-sourced	0	FAIL
AX-2026-043727	BCH-2602-E8475	Janoshik	2026-Q3	98.00	8.79	—	Supplier-supplied	0	FAIL
AX-2026-043728	BCH-2602-E8475	Medutest	2026-Q3	97.72	9.38	97.5	Buyer-sourced	1	FAIL
AX-2026-043729	BCH-2602-E8475	Janoshik	2026-Q3	98.14	9.23	97.2	Supplier-supplied	0	FAIL
AX-2026-043730	BCH-2602-E8475	PeptideMeter	2026-Q3	98.29	9.18	—	Buyer-sourced	0	FAIL
AX-2026-043731	BCH-2602-E8475	Janoshik	2026-Q3	97.54	8.23	88.9	Buyer-sourced	1	FAIL
AX-2026-043733	BCH-2602-E8475	Janoshik	2026-Q3	97.42	9.41	—	Buyer-sourced	0	FAIL
AX-2026-043734	BCH-2602-E8475	Janoshik	2026-Q3	98.16	9.35	—	Supplier-supplied	0	FAIL
AX-2026-043735	BCH-2602-E8475	PeptideMeter	2026-Q3	97.75	9.05	99.6	Buyer-sourced	0	FAIL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent N-Acetyl Semax Amide records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043498	CPC	CPC-2602-D3496	VendorInvestigate	2026-Q3	98.23	6.05	102.8	Buyer-sourced	1	FAIL
AX-2026-043726	BCH	BCH-2602-E8475	Janoshik	2026-Q3	97.89	9.67	104.2	Buyer-sourced	0	FAIL
AX-2026-043497	CPC	CPC-2602-D3496	Janoshik	2026-Q3	98.59	5.62	99.9	Buyer-sourced	0	PASS
AX-2026-043495	CPC	CPC-2602-D3496	Janoshik	2026-Q3	98.86	5.63	—	Buyer-sourced	0	PASS
AX-2026-043729	BCH	BCH-2602-E8475	Janoshik	2026-Q3	98.14	9.23	97.2	Supplier-supplied	0	FAIL
AX-2026-043734	BCH	BCH-2602-E8475	Janoshik	2026-Q3	98.16	9.35	—	Supplier-supplied	0	FAIL
AX-2026-043731	BCH	BCH-2602-E8475	Janoshik	2026-Q3	97.54	8.23	88.9	Buyer-sourced	1	FAIL
AX-2026-043496	CPC	CPC-2602-D3496	Nordanalyt	2026-Q3	99.04	6.03	—	Supplier-supplied	0	PASS

Other recent BCH records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043691	BPC-157	BCH-2603-L7768	Janoshik	2026-Q3	98.66	5.12	—	Supplier-supplied	1	MARGINAL
AX-2026-043693	BPC-157	BCH-2603-L7768	PeptideMeter	2026-Q3	97.82	5.21	94.9	Buyer-sourced	0	FAIL
AX-2026-043686	BPC-157	BCH-2603-L7768	VendorInvestigate	2026-Q3	97.64	5.27	—	Buyer-sourced	0	FAIL
AX-2026-043695	BPC-157	BCH-2603-L7768	Janoshik	2026-Q3	97.61	5.42	—	Buyer-sourced	0	FAIL
AX-2026-043726	N-Acetyl Semax Amide	BCH-2602-E8475	Janoshik	2026-Q3	97.89	9.67	104.2	Buyer-sourced	0	FAIL
AX-2026-043688	BPC-157	BCH-2603-L7768	Janoshik	2026-Q3	97.94	5.56	—	Buyer-sourced	0	FAIL
AX-2026-043658	BPC-157	BCH-2603-G6644	Janoshik	2026-Q3	98.45	4.37	101.8	Supplier-supplied	0	PASS
AX-2026-043692	BPC-157	BCH-2603-L7768	VendorInvestigate	2026-Q3	97.60	5.61	—	Buyer-sourced	0	FAIL