AssayIndex
Chromatography

Gradient

Programmed change in mobile-phase composition during a run. Gradient duration determines resolving power and is therefore part of the result, not metadata.

Related terms

Gradient slope
Percentage points of organic solvent per minute. Lower slope, better resolution, longer run.
see also Gradient
Isocratic
Constant mobile-phase composition throughout the run. Simpler, and unsuitable for samples spanning a wide hydrophobicity range.
see also Gradient

Where this term appears

The glossary exists to make record pages readable without a chemistry degree. Every technical term on a report resolves here.