AssayIndex
Chromatography

Baseline

The detector signal in the absence of an eluting analyte. On a gradient it drifts, and where the baseline is drawn determines the integrated area.

Related terms

Peak integration
Determining a peak's area by defining its start, end and baseline. The step where a purity figure is actually decided, and the one least often documented.
Drift
Slow monotonic change in the baseline across a run. Caused by mobile-phase absorbance changing with organic fraction, or by column temperature not being controlled.
see also Baseline

Where this term appears

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