AssayIndex
Chromatography

Retention time

Time from injection to peak apex. A property of the compound, the column and the gradient — comparable across laboratories only after gradient normalisation.

Related terms

Gradient normalisation
Converting a retention time measured on one gradient duration to the index reference scale, so retentions from different laboratories can be compared.
Rule AX-R11
Retention time outside the compound window after gradient normalisation. Two thirds of instances are undocumented method differences.

Where this term appears

The glossary exists to make record pages readable without a chemistry degree. Every technical term on a report resolves here.