Wavelength, detection
The UV wavelength at which absorbance is measured. 214 nm sees the amide backbone; 280 nm sees only aromatic residues. Purity figures at the two are not comparable.
Related terms
- Photodiode array (PDA)
- A UV detector acquiring a full spectrum at every time point rather than one or two wavelengths. Enables peak-purity assessment and the 280/214 ratio.
- Chromophore
- The part of a molecule responsible for UV absorbance. In peptides: the amide bond below 230 nm, and Trp/Tyr near 280 nm.
see also Peak purity
see also Wavelength, detection
Where this term appears
The glossary exists to make record pages readable without a chemistry degree. Every technical term on a report resolves here.
- Assay report records — the term appears in result tables, peak tables and provenance blocks
- Method references — where the measurement behind the term is defined
- All 2 glossary terms beginning with W