AssayIndex
Chromatography

Peak integration

Determining a peak's area by defining its start, end and baseline. The step where a purity figure is actually decided, and the one least often documented.

Related terms

Baseline
The detector signal in the absence of an eluting analyte. On a gradient it drifts, and where the baseline is drawn determines the integrated area.
Drop-line integration
Separating two overlapping peaks with a vertical line dropped from the valley between them. Unbiased when the peaks are of comparable size.
Reporting threshold
The area percent below which peaks are not reported. The index uses 0.05 %. Excluded peaks are excluded from the total, so purity is systematically overstated by their sum.

Where this term appears

The glossary exists to make record pages readable without a chemistry degree. Every technical term on a report resolves here.