Reverse-phase chromatography
Separation on a non-polar stationary phase, typically C18-bonded silica, with a polar mobile phase. Retains by hydrophobic interaction.
Related terms
- HILIC
- Hydrophilic-interaction liquid chromatography. Polar stationary phase, high-organic mobile phase. Retains polar analytes that reverse phase cannot hold.
- C18
- Octadecyl-bonded silica: the standard reverse-phase stationary phase for peptides. Retention scales with the analyte's exposed hydrophobic surface, not with its mass.
see also Reverse-phase chromatography
Where this term appears
The glossary exists to make record pages readable without a chemistry degree. Every technical term on a report resolves here.
- Assay report records — the term appears in result tables, peak tables and provenance blocks
- Method references — where the measurement behind the term is defined
- All 37 glossary terms beginning with R