AX-2026-043619 · BPC-157 · WXT lot WXT-2602-W5087

Section 1

Results

Measured against the index acceptance specification for the tissue-repair and immunomodulatory peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 32 min gradient, 214 nm	97.99	% area	≥ 98.0	FAIL
Total impurities	RP-HPLC, sum of reported peaks	2.01	% area	≤ 2.0	FAIL
Largest single impurity	RP-HPLC — truncated (1-12)	0.91	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	1418.7093	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, high resolution (ESI-TOF/QTOF)	1418.7104	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	+0.8	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	89.13	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	87.34	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	5.88	mg	5 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	5.136	mg	5 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	102.7	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	4.68	%	≤ 10.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	5.78	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.41	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear with a single visible fibre	—	clear, no visible particulates	FAIL
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	4.23	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 32 min gradient	10.26	min	10.21 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.07	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	66,955	—	≥ 20,000	PASS
Resolution to nearest impurity	vs [D-Val]-diastereomer	0.92	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 32 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 66,955, and skew follows the reported asymmetry factor of 1.07. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.91 % impurity against a 97.99 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	BPC-157 — main peak	10.26	4,762,618	97.99	—
2	truncated (1-12)	9.24	44,229	0.91	-285.132
3	acetylated N-terminus (+42 Da)	10.77	29,162	0.60	+42.011
4	des-Gly truncate (−57 Da)	9.90	13,609	0.28	-57.021
5	[D-Val]-diastereomer	10.11	6,804	0.14	0.000
6	pyroglutamate cyclisation (−17 Da)	10.59	2,430	0.05	-17.027
7	BPC-157 arginine-salt artefact	9.67	1,458	0.03	0.000
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 1418.7093 Da, computed from C62H98N16O22. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 1418.7104 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
3+	473.9104	473.9107	0.0003	7.7
2+	710.3619	710.3625	0.0006	51.8
1+	1419.7166	1419.7177	0.0011	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	4.68
Counter-ion (acetate)	ion chromatography	5.78
Residual solvent + inorganic residue	estimated	0.41
Total peptide content	by difference	89.13
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	98.99	97.99	divergence +0.51 pp vs batch median
Total impurities (% area)	1.01	2.01
Largest single impurity (% area)	0.64	0.91
Water (%)	4.7	4.68
Counter-ion (%)	6.6	5.78
Peptide content (%w/w)	98.7	89.13
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	NO	—	no method section — the result cannot be reconciled against any other

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (ambient, 29 d) → laboratory (hand-carried, 2 d) → assay.
Assay date: 10 Jun 2026
Submitted to the index: 26 Jun 2026
Laboratory: VendorInvestigate, Riga, Latvia
Method basis: 32 min gradient · high-resolution MS
Verification status: VERIFIED

What this report does not establish

That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 4

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

lambda_28026 Jun 2026

97.99 % on BPC-157 is below the index specification for the class. The interesting part is the distribution: truncated (1-12) at 0.91 % is doing most of the work, and that species is a synthesis artefact rather than a degradation product. This is a process observation about lot WXT-2602-W5087, not a storage one.

des_gly26 Jun 2026

truncated (1-12) at 0.91 % points at an incomplete coupling rather than at decomposition. Deletion sequences do not form in a vial; they form on a resin, and they are fixed by a double coupling rather than by a colder courier.

price_per_mg26 Jun 2026

A retail purchase carries a price, which makes cost per milligram of recovered peptide computable. That is the comparison a buyer actually needs and it exists only for this provenance class.

sample_vs_retail26 Jun 2026

The index cannot verify that the vial assayed is representative of the lot it came from, and says so on every record. Provenance narrows that gap; it does not close it.

Section 7

Related records

Same batch — lot WXT-2602-W5087 n=3

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043617	WXT-2602-W5087	Janoshik	2026-Q2	98.48	5.28	—	Supplier-supplied	1	MARGINAL
AX-2026-043618	WXT-2602-W5087	PeptideMeter	2026-Q2	98.54	4.74	98.7	Supplier-supplied	2	MARGINAL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent BPC-157 records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043691	BCH	BCH-2603-L7768	Janoshik	2026-Q3	98.66	5.12	—	Supplier-supplied	1	MARGINAL
AX-2026-043493	CPC	CPC-2602-W3201	Janoshik	2026-Q3	98.76	2.27	—	Buyer-sourced	2	FAIL
AX-2026-041387	WWB	WWB-2606-H3906	Janoshik	2026-Q3	99.33	4.64	—	Laboratory-retained	0	PASS
AX-2026-043693	BCH	BCH-2603-L7768	PeptideMeter	2026-Q3	97.82	5.21	94.9	Buyer-sourced	0	FAIL
AX-2026-043686	BCH	BCH-2603-L7768	VendorInvestigate	2026-Q3	97.64	5.27	—	Buyer-sourced	0	FAIL
AX-2026-043695	BCH	BCH-2603-L7768	Janoshik	2026-Q3	97.61	5.42	—	Buyer-sourced	0	FAIL
AX-2026-043487	CPC	CPC-2602-W3201	VendorInvestigate	2026-Q3	99.07	2.76	97.0	Laboratory-retained	1	MARGINAL
AX-2026-041385	WWB	WWB-2606-H3906	Medutest	2026-Q3	99.29	4.43	—	Buyer-sourced	0	PASS

Other recent WXT records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043651	Pramlintide	WXT-2603-D6459	PeptideMeter	2026-Q3	98.21	4.97	101.6	Buyer-sourced	1	MARGINAL
AX-2026-043531	Leuprolide	WXT-2604-K4483	Medutest	2026-Q3	99.00	5.03	—	Buyer-sourced	0	PASS
AX-2026-043561	Semaglutide	WXT-2604-C5135	Janoshik	2026-Q3	96.96	4.22	91.3	Buyer-sourced	0	FAIL
AX-2026-043627	L-Glutathione (reduced)	WXT-2605-B5720	Janoshik	2026-Q3	97.96	6.08	92.3	Buyer-sourced	1	FAIL
AX-2026-043564	Semaglutide	WXT-2604-C5135	Janoshik	2026-Q3	97.15	4.44	104.7	Buyer-sourced	0	PASS
AX-2026-043533	Leuprolide	WXT-2604-K4483	PeptideMeter	2026-Q3	99.89	4.94	—	Supplier-supplied	0	PASS
AX-2026-043624	L-Glutathione (reduced)	WXT-2605-B5720	Nordanalyt	2026-Q3	98.18	6.95	105.3	Buyer-sourced	2	FAIL
AX-2026-043556	Semaglutide	WXT-2604-C5135	PeptideMeter	2026-Q3	97.56	4.35	89.6	Buyer-sourced	1	MARGINAL